MS Wil GmbH

P.O. Box 1426  - CH 8048 Zurich
Phone +41 71 912 12 77
Fax +41 71 912 12 79
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Cut consumables costs and use fused silica capillary columns on your Thermo Easy-spray source 

Closeup of the EASY-SPRAY jailbreak

--> NO MODIFICATIONS REQUIRED TO YOUR EASY-SPRAY SOURCE

Easy-Spray Jailbreak  - designed by Phoenix S&T - enables researchers to use standard 360um OD fused silica columns on their Thermo Easy-Spray, regaining full control over the column setup again. There are good reasons to standardize and simplify a NanoLC setup. In certain cases your Protein IDs or the observed spray stability may not satisfy you. The consumables cost burden may also be a motivation. Then, Easy-Spray jailbreak might be your solution.

The heater (blue color) restores the freedom of taking control over column length, emitter size, use of DiY and/or selfpack columns. Our partner company New Objective has a large set of NanoLC solutions for your demanding research. [PDF]

Jailbreak uses the thermal control electronics you already have integrated beneath your Easy-Spray source - same sensor - same precision. The heater assembly itself is spec`d to 100°C, however the Texas instruments LM62 temperature sensor picked by Thermo rates 90°C maximum.

If you are really curious about the embedded temperature sensor, here are the specs: 

http://www.ti.com/lit/ds/symlink/lm62.pdf

The white HV cable carries the ESI voltage out of the source and guides it to the column inlet connection. This way, post column peak broadening issues are circumnavigate, enabling maximum possible peak capacity and peak concentration. The original Easy Spray columns feature post column HV feed in, which might be a compromise as it preserves the option to swap the emitter while one can still use the column. - But is this the case on your Easy spray column?  

A fundamental desire is maximum peak capacity and instrumental sensitivity, which translates to better proteine IDs.

As ESI depicts a concentration dependent form of ion generation, narrow peaks are key.

Why use Picofrit 

Jailbreak can be ordered at any length between 12 and 25cm (in 1cm increments)

 Part number   Description  net price €
 PST-ESR-xx  Easy-spray heater with HV cable only      2.500 
 PST-ESR-xxU
 Easy-spray heater with HV cable and stainless union
     2.900 
 PST-ESR-xxT  Easy-spray heater with HV cable and Tee  
     2.900 

Ask for a set with column packing devices from Western Fluids, or Corsolutions!

See what EASY-Spray Jailbreak looks like on guide me to the pictures

 EASY-SPRAY Jailbreak column oven   above:  PST jailbreak on a Thermo Fusion equipped with a Easy-Spray source.

The Upchurch T-piece provides high voltage

Jailbreak Spray and needle video still

 above:  PST jailbreak - what it looks like with the Thermo Video camera

 

pick the Jailbreak brochure PDFpick the Jailbreak manual PDF

Jailbreak at Uni Lausanne

 

Many Thanks to Toni from Columbia University  and Manfredo Quadroni from Uni Lausanne

for testing and pictures of EASY-Spray Jailbreak

http://www.hiccc.columbia.edu/research/sharedresources/proteomics

http://www.unil.ch/cig/en/home/menuinst/research/core-facilities/dr-quadroni---paf.html

 

Jailbreak at Erasmus 1Jailbreak at Erasmus 2

Ing. Karel Bezstarosti,  Proteomics specialist, Senior research technician
This email address is being protected from spambots. You need JavaScript enabled to view it.
phn:  +31 10 7038124

Reference: http://www.proteomicscenter.nl/people.html

 


 

FAQs:

1.: What is the minimum length of an emitter when I use Jailbreak?

A.: The jailbreak device is 8 cm including the ferrule keeping the emitter in position. This is the lenght without a build in Pencilheater unit. So a minimum emitter lenght of 10 cm or above is practical


 

2.: How do I feed in the high voltage (HV) ?

A.: There are various options available. Below are pictures of two different magnetically attachable HV connections. IDEX ferrule on the left and a IDEX T - housing  - both with magnetic connectors designed by PST. The last letter of the product ID encodes the HV, e.g. PST-acc-U and PST-acc-T.


 

 

IDEX UnionIDEX T-union with Platinium wire


3.: I am a Thermo NanoViper lover - What is the solution? 

A.: We have reducing unions, enabling you to connect male NanoViper to 360um fused silica. Order code is -RU