Rapid, Semi-automated Immunopeptidome profiling

Rapid, Semi-automated Immunopeptidome profiling

In 2022 MS Wil has also become supplier of ReSyn Biosciences’ MagReSyn microparticles. These microparticles are build up on a completely new microparticle technology platform. ReSyn’s new technology comprises a hyper-porous polymer matrix that allows penetration of biological and synthetic molecules throughout the volume of the microparticles. This offers exceptionally high surface area for binding of molecules and allows performance that is orders of magnitude greater than alternate technologies. MagReSyn microparticles are therefore very suitable for the development of new methods for achieving high biomolecule binding capacity (enabling miniaturization and high-throughput processes), for different kinds of applications. In that light,…
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Finding alternatives for Self-pack columns

Finding alternatives for Self-pack columns

Many of our customers are used to use the New Objective PicoFrit or SilicaTip emitters to pack their own “integrated-tip” nanoLC columns. However, since these emitters have been hard to get recently, some rethinking is dearly needed. Self-Pack columns with integrated emitters (also known as pulled emitters) are fused silica lines often with an Outer Diameter (OD) of 360µm, an Inner Diameter (ID) of 50-100µm (depending on the flow rate), and a pulled tip between 8 and 30µm ID. In the case of the PicoFrit, a frit is packed in the pulled tip, as shown in the drawing below (figure…
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The Complete Solution for Native MS

The Complete Solution for Native MS

Curious about a way to achieve high sensitivity, high throughput, and high quantitation accuracy for Native MS research using the MnESI platform? In this article, we provide you with a solution to achieve all this while addressing the main challenges of Native MS. Native MS maintains a biomolecule’s natural folded state and associated non-covalent interactions for mass spectrometry analysis and is a powerful technique for studying the structure of intact proteins, large protein complexes, and protein-protein, protein-ligand interactions. Currently, one of the biggest challenges for native MS is the analysis of large native protein complexes and their mixtures in a…
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Mounting your Butterfly or Pencil Heater Column Oven on your Thermo Ion Source

Mounting your Butterfly or Pencil Heater Column Oven on your Thermo Ion Source

Over the years MS Wil has provided many Proteomics laboratories with solutions to build column ovens close to the orifice of the Mass Spectrometer. Outside bespoke solution from Sonation, these ovens are often the different versions of Butterfly and Pencil Heaters made by Phoenix S&T. Where the Sonation oven is build to be mounted on the XYZ-manipulator arm of the Thermo Nanospray Flex Ion™ Source, there has been no real solution to mount the Phoenix products to the Thermo Nanospray Flex Ion Source, let alone the EasySpray source. In this product note we describe 3 new ways to mount the…
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Self Pack Columns with(out) Frit using Integrated/Pulled Emitters

Self Pack Columns with(out) Frit using Integrated/Pulled Emitters

As many of our customers pack their own pulled emitter columns, MS Wil provides several options to fulfil this need. Today we provide several Self Pack columns, with different Inner Diameters (ID’s) and with or without integrated frits. First some nomenclature: Pulled emitters and Integrated emitter columns are different words for the same thing. For the remainder of this article, we will use Integrated emitter columns, just because it is a more generic name for the column type. Emitters of 75-100µm ID with a length of 50cm and a narrow tip are popular columns to be packed with your material…
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What you need to know about the ABIRD (Active Background Ion Reduction Device)

What you need to know about the ABIRD (Active Background Ion Reduction Device)

ABIRD (or Active Background Ion Reduction Device) is a popular accessory for nanospray-powered mass spectrometers, and has been approved for use on all Thermo Scientific instruments and nanospray sources. ABIRD will consistently: Isolate the instrument from spikes in lab air, which is critical for extended quantitative or comparative analyses. Reduce background ion signals in full MS during nanospray-ESI analysis, enhancing S/N ratio quite dramatically in many cases. These are benefits that only an ABIRD can provide, making it a smart enhancement for any nanospray ESI/LC-MS system. Many users hope to demonstrate improved peptide detection with ABIRD installed, but get frustrated…
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New Products

New Products

Since it is our goal to be a one-stop-shop for fine Mass Spectrometry Tools, we are constantly searching for new products to extend our portfolio. Even during the Summer Holidays we have been busy looking for new solutions to optimize your research, and help Make your Science Work. Therefore, we would like to present to you an overview of our new products: Aurora UHPLC Columns IonOpticks produces high-performance nanoflow chromatography solutions, including their high-performance and user-friendly Aurora UHPLC Columns. Delivering unrivalled coverage, throughput, sensitivity and reproducibility, Generation 3 Aurora SeriesTM columns embody all of the strengths of previous generations, augmented by…
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Make near perfect glass capillary ends, using the Capillary Polishing Station

Make near perfect glass capillary ends, using the Capillary Polishing Station

Looking for a way to make near perfect glass capillary ends in the lab, to give optimal chromatographic response and robustness? The new and improved Capillary Polishing Station 2 (CPS-2) from ESI Source Solutions allows you to do so. This device provides a simple means to make clean polished ends on various OD glass capillaries tubing and 1/16” PEEK tubing as well. This is essential for reliable high-sensitivity capillary chromatography. The device consists of two parts, the polishing base and the capillary jig, shown below (see figure 1). Figure 1. Capillary Polishing Station Package (incl. polishing base, capillary jig, and…
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Making Meaningful nLC Connections

Making Meaningful nLC Connections

Often our customers ask us: “How do I make my connections work in my nanoLC-MS set-up?” or a variant of this phrase. In this article we will provide you with our insight into this misty territory, with many options and pitfalls. The first question for you to answer is: “What do I want to connect and with what purpose”. Is it “just” a coupling between a transfer line and a column or emitter, or do you want to turn the connection into a Liquid Junction at the same time. In latter case, you may wish to look into some electrical…
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