|Bead Size (um)|
MagReSyn Protein A (2 ml)
MagReSyn Protein A (2 ml)
MagReSyn® Protein A microparticles contain covalently coupled Protein A for highly specific single-step purification of IgG to >95% purity from whole serum samples. Protein A is a ~56 kDa surface protein originally isolated from the cell wall of the bacterium Staphylococcus aureus and is widely used for highly specific purification of IgG antibodies and for immunoprecipitation experiments. Protein A has 5 immunoglobulin binding domains folded into a three-helix bundle and specifically binds the Fc region on the heavy chain of the IgG class of antibodies. More specifically, Protein A binds with high affinity to specific subclasses of human, rabbit, mouse, guinea pig, cat and pig IgG. It does not react with human IgG3, IgD or IgE, mouse IgM, IgA or IgE, or goat, koala and llama IgG. The high recombinant Protein A content of MagReSyn® ensures a high immunoglobulin capacity allowing for experimental miniaturization.
Interesting Application Note on Rapid, semi-automated immunopeptidome profiling (using MagReSyn Protein A). Click on the image below for the full application note.
New miniaturized, high throughput technologies for bioseparation, diagnostics, DNA sequencing, flow cytometry, drug discovery, proteomics and genomics are in many instances reliant on attachment of functional biological molecules to a microsphere support. The vast array of life sciences applications include: capture reagents for immunoassay (fluorescence, enzyme linked etc); surfaces for immunoprecipitation; diagnostic assays; fluorescence microscopy; flow/imaging cytometry; magnetic cell separation; molecular diagnostics; agglutination tests; nucleic acid separation and protein separation among others.
Conventional microparticle technologies use solid or porous/cracked microparticles with binding of biological molecules limited to the surface, a key factor constraining performance and the development of new applications for microparticle technologies and products. We have developed a novel (patented) microparticle technology platform, comprising a hyper-porous polymer matrix that allows penetration of biological and synthetic molecules throughout the volume of the microparticles. This offers exceptionally high surface area for binding of molecules and allows performance that is orders of magnitude greater than alternate technologies. The binding capacity serves as a major performance contributor to the number of applications and versatility for end-user applications by and further enables miniaturization, increased sensitivity and reducing the cost of R&D.