MagReSyn Ti-IMAC HP (2 ml; Max 6mnd Shelf-life)
Protein phosphorylation is one of the most important post-translational modifications and is a critical process in cellular signaling and regulation of cellular networks. Comprehensive analysis of the phosphoproteome is a challenging task due to the transient and sub-stoichiometric nature of phosphorylation sites. High-throughput phosphoproteome analysis by mass spectrometry requires compatible technologies than can specifically enrich phosphopeptides. MagReSyn® Ti-IMAC microparticle have a flexible linker (to reduce steric hindrance) activated with phosphonate groups for Ti4+ chelation. The unique properties of the proprietary ReSyn microparticle technology allows extremely specific, reproducible enrichment of phosphopeptides from complex biological samples/protein digests. The microparticles can be used either alone, or in combination with MagReSyn® TiO2, MagReSyn® Zr-IMAC and/or MagReSyn® ZrO2 to increase phosphoproteome coverage.

MagReSyn Technology Description

New miniaturized, high throughput technologies for bioseparation, diagnostics, DNA sequencing, flow cytometry, drug discovery, proteomics and genomics are in many instances reliant on attachment of functional biological molecules to a microsphere support. The vast array of life sciences applications include: capture reagents for immunoassay (fluorescence, enzyme linked etc); surfaces for immunoprecipitation; diagnostic assays; fluorescence microscopy; flow/imaging cytometry; magnetic cell separation; molecular diagnostics; agglutination tests; nucleic acid separation and protein separation among others.

Conventional microparticle technologies use solid or porous/cracked microparticles with binding of biological molecules limited to the surface, a key factor constraining performance and the development of new applications for microparticle technologies and products. We have developed a novel (patented) microparticle technology platform, comprising a hyper-porous polymer matrix that allows penetration of biological and synthetic molecules throughout the volume of the microparticles. This offers exceptionally high surface area for binding of molecules and allows performance that is orders of magnitude greater than alternate technologies. The binding capacity serves as a major performance contributor to the number of applications and versatility for end-user applications by and further enables miniaturization, increased sensitivity and reducing the cost of R&D.