MagReSyn Trypsin (5 ml)
MagReSyn® Trypsin microparticles consist of covalently immobilized sequencing grade trypsin (TPCK treated to prevent chymotryptic activity) for protein digestion prior to mass spectrometry analysis. The porous high capacity MagReSyn® microparticle technology allows for high volumetric enzyme activity allowing for rapid digestion. The magnetically immobilized trypsin is stabilized against denaturation and retains functionality under denaturing conditions (including the presence of urea and SDS) with limited trypsin autolysis, allowing for new automated and miniaturized digestion workflows, improving digestion reproducibility.

MagReSyn Technology Description

New miniaturized, high throughput technologies for bioseparation, diagnostics, DNA sequencing, flow cytometry, drug discovery, proteomics and genomics are in many instances reliant on attachment of functional biological molecules to a microsphere support. The vast array of life sciences applications include: capture reagents for immunoassay (fluorescence, enzyme linked etc); surfaces for immunoprecipitation; diagnostic assays; fluorescence microscopy; flow/imaging cytometry; magnetic cell separation; molecular diagnostics; agglutination tests; nucleic acid separation and protein separation among others.

Conventional microparticle technologies use solid or porous/cracked microparticles with binding of biological molecules limited to the surface, a key factor constraining performance and the development of new applications for microparticle technologies and products. We have developed a novel (patented) microparticle technology platform, comprising a hyper-porous polymer matrix that allows penetration of biological and synthetic molecules throughout the volume of the microparticles. This offers exceptionally high surface area for binding of molecules and allows performance that is orders of magnitude greater than alternate technologies. The binding capacity serves as a major performance contributor to the number of applications and versatility for end-user applications by and further enables miniaturization, increased sensitivity and reducing the cost of R&D.