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S-Trap 96-Well Turbo Mini Plate (P003-96MNT-0003PL)

S-Trap 96-Well Turbo Mini Plate (P003-96MNT-0003PL)

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S-Trap 96-Well Turbo Mini Plate (P003-96MNT-0003PL)

S-Trap™ Turbo 96-Well Plate enhances throughput by matching sample prep speed with new fast detection technologies. It minimizes elution volumes, delivering highly concentrated peptides ready for immediate LC-MS or MALDI analysis, eliminating the dry-down step! The S-Trap™ Turbo handles diverse sample types without protocol modification.

Unit volume: 2 mL
Preparations: 96 Samples x 100-300 μg

S-Trap

S-Trap sample processing technology is convenient spin columns or automatable 96-well plates that enable the use of SDS in proteomics sample preparation. SDS is a nearly universal protein solvent that dissolves even poorly-soluble molecules like membrane proteins which are often discarded as pellets. S-Trap sample processing begins with sample lysis and solubilization in 5% SDS. Proteins are further denatured by acidification to pH < 1 and subsequent exposure to a high concentration of methanol. This three-stage denaturation ensures complete destruction of undesired enzymatic activity such as proteases and phosphatases, and maximizes efficiency of downstream digestions. Reduction and alkylation can be performed in 5% SDS, precluding precipitation, or alternatively on-column after the denatured proteins are trapped.