Tryp-N Kit (K01-20)

Includes optimized digestion buffer.

Tryp-N^™

Tryp-N^™ is a thermophilic metalloprotease with N-terminal specificity for arginine and lysine engineered at Cold Spring Harbor Laboratory (CSHL). Tryp-N^™ provides clarified spectra and greater sensitivity by yielding peptides in which the basic centers of peptides – the amino terminus and side chains of K/R –  are located together to produce b-ion dominated spectra. Tryp-N has been demonstrated to yield simplified MS/MS fragmentation and have the best proteolytic activity.¹ Tryp-N spectra provide the complimentary ion series to compliment trypsin, allowing you to more easily find and specifically localize PTMs. PTM identification and localization of PTMs, such as phosphorylation in YST clusters, and de novo sequencing. Additionally, as ion current remains on b-ions during fragmentation of MRM assays, rather than becoming diluted between two charged termini in tryptic peptides, sensitivity is often enhanced.