Universal proteomics sample preparation kit, 96 well plate (100 µg – 300 µg) with solutions (K02-96-1)

96-well plate S-Trap sample processing in a full kit format for samples from 100 µg – 300 µg. The kit includes a 96-well S-Traps plate, a waste plate, an elution plate and plate seal, and lysis, wash and digestion buffers. The user must supply HPLC or MS grade MeOH and their choice of protease.

S-Trap^™

S-Trap^™ sample processing technology is convenient spin columns or automatable 96-well plates that enable the use of SDS in proteomics sample preparation. SDS is a nearly universal protein solvent that dissolves even poorly-soluble molecules like membrane proteins which are often discarded as pellets. S-Trap^™ sample processing begins with sample lysis and solubilization in 5% SDS. Proteins are further denatured by acidification to pH < 1 and subsequent exposure to a high concentration of methanol. This three-stage denaturation ensures complete destruction of undesired enzymatic activity such as proteases and phosphatases, and maximizes efficiency of downstream digestions. Reduction and alkylation can be performed in 5% SDS, precluding precipitation, or alternatively on-column after the denatured proteins are trapped.