Universal proteomics sample preparation kits, micros (≤ 100 µg) with solutions (K02-micro-10)

S-Trap micro sample processing in a full kit format for samples ≤ 100 µg. The kit includes S-Trap micros, extra tubes, and lysis, wash and digestion buffers. The user must supply HPLC or MS grade MeOH and their choice of protease.

S-Trap^™

S-Trap^™ sample processing technology is convenient spin columns or automatable 96-well plates that enable the use of SDS in proteomics sample preparation. SDS is a nearly universal protein solvent that dissolves even poorly-soluble molecules like membrane proteins which are often discarded as pellets. S-Trap^™ sample processing begins with sample lysis and solubilization in 5% SDS. Proteins are further denatured by acidification to pH < 1 and subsequent exposure to a high concentration of methanol. This three-stage denaturation ensures complete destruction of undesired enzymatic activity such as proteases and phosphatases, and maximizes efficiency of downstream digestions. Reduction and alkylation can be performed in 5% SDS, precluding precipitation, or alternatively on-column after the denatured proteins are trapped.