FTMS Booster X1 gives your existing Thermo Orbitrap (… & Thermo FTICRs) a quantum boost in data quality and also in processing sophistication. In order to help you to add a fresh breeze in performance to your mass spec so that you can unravel your most complex and challenging applications on todays instruments.
The device improves sensitivity, lowers limit of detection and limit of quantitation owing to…
- Full Transient Recording
- On-line Absorption Mode FT
- Full Profile Mode Mass Spectra
- Reduced Analog and also Digital Noise
What is FTMS Booster X1?
FTMS Booster X1 is a high-performance data acquisition and analysis system based on state-of-the-art FPGA (field-programmable gate array) technology. Together with Peak by Peak™ software, FTMS Booster X1 is a complete state-of-the-art solution for acquisition of time-domain signals (transients), real-time digital signal processing, trigger decoding, and off-line data visualization and processing.
Compatible with any Orbitrap™ FTMS platform (LTQ Orbitrap™, Exactive™, and Fusion™ series), the device features both on-line and off-line software operation in parallel to installed products. Two complementary data sets are simultaneously and synchronously acquired. Both Booster-produced and conventional transients or mass spectra can be processed by Peak by Peak™ and AutoVectis™ software tools. Booster data is synchronized with metadata that is produced by the existing software.
In conclusion, the device gives your existing instrument a quantum boost in data quality and processing sophistication. In order to help you to take on the most complex and challenging applications on your instrument.
How does the FTMS Booster X1 work?
High-throughput in-line FPGA technologies are the basis of the new architecture in FTMS Booster X1. Compared to traditional systems, the fidelity, accuracy and extent of the acquired time-domain data are substantially improved. In conclusion this results directly into improved analytical performance.
- Reduced Noise – which translates to improved sensitivity
- Dynamic Range
- Full Transient Recording for Improved Resolution
- Reduced Phase Distortions for Improved Resolution
- Mass Accuracy
How can I implement the FTMS Booster X1?
Parallel, simultaneous acquisition and processing paths maximize instrument and also analysis flexibility.
Why is it good for my application?
The FTMS Booster X1 increases sensitivity, limit of detection (LOD), and also limit of quantitation (LOQ) in comparison with standard built-in data acquisition systems for Orbitrap™ FTMS applications, including proteomics. Take a look at sensitivity, LOD, LOQ performance with Orbitrap™ QExactive Plus™ FTMS (Thermo Scientific) with and without FTMS Booster X1.
Panel (A) shows a total ion current (TIC) chromatogram, panels (B) and (C) each show mass spectra from LC MS / MS experiment of 6×5 reference peptide mix (Promega). The data are represented in (black) enhanced FT (eFT, Thermo Scientific) and (red) absorption mode FT (aFT, AutoVectis).
Imagine you are working on a TMT 10 plex (Tandem Mass Tag™) experiment designed in order to enable identification and quantitation of proteins in different samples using tandem mass spectrometry (MS) e.g. a kit from Thermo. TMT10plex Label Reagents share an identical structure with TMTzero, TMTduplex, and TMTsixplex reagents but contain different numbers and combinations of 13C and 15N isotopes in the mass reporter. Resolving the low mass region and preserving may soon show the limits of todays Orbitrap electronics, as they reduce and fiddle on raw data so that the post processing systems can deal with the data stream.
Blue lines illustrate FTMS Booster X1 compared to original black dotted line generated by Thermos original data processing. Hence, it becomes obvious that the resolving power is useful. Experiments probing the effect on TMT ratios also show dramatic improvements. Details are explained in ASMS 2016 poster “Implementation of a high-performance FPGA-based data aquisition system for FTMS”
Posters from ASMS 2016:
Boosting FTMS performance via advanced data acquisition electronics and signal processing
Implementation of a high-performance FPGA-based data acquisition system for FTMS
Further information as PDF:
FTMS Booster X1 for Orbitrap FTMS
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FTMS Booster X1 for FT-ICR MS
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