Pack your own LC/MS Capillary Columns and get better results!
Pressure Injection Cells
Load your samples into the mass spectrometer without transfer loss or contact with metallic surfaces.
These Column Packers ~ or Pressure Injection Cells as they are also called enable controlled dispensing of small-volume liquid samples. Using high pressure, the pressure injection cells have proved invaluable for two applications: densely packing nanobore capillary columns with solid-phase particles; and precisely infusing microliter samples directly from microcentrifuge tubes into mass spectrometers without additional transfers, wasted sample, or contact with metallic surfaces which adsorb some negatively charged molecules such as peptides with phospho or carboxy groups.
The Pressure Injection Cell holds 1 mL and 2 mL micro-centrifuge tubes as well as 12 x 32 mm glass vials in its central chamber. The assembly can be used on a typical magnetic stirrer, e.g. to keep particles in suspension. The Pressure Injection Cells accept various sample tubes, including 0.5 mL, 1.5 mL, and 2.0 mL microcentrifuge tubes and 12 mm diameter glass vials. The cell needs to be connected to a bottle/tank of compressed gas, such as Helium or Nitrogen. Packing capillary columns requires several hundred to about 1000 psi or sometimes more, and mass spectrometer injections typically require a few hundred psi. This unit is rated for a 2500 psi. 8500 psi units are available.
Features:
- Easy sample tube removal since the tube protrudes above the bottom section of the pressure injection cell.
- O-ring seal at interface ensures no leakage.
- Includes a 3-way valve for easy switching between pressurization and release of pressure.
- Three bolt system secures the cell, allows easy addition and removal of tubes / vials.
- Holds 0.5 to 2 ml microcentrifuge/Eppendorf tubes and 12 x 32 mm (glass) vials.
- Includes operator’s manual; 10 reusable ferrules for typical (380 µm outside diameter) capillaries (other ferrules are available); and 1.5 mL of Kasil and 0.5 mL of formamide and a cleaving tool for making your own frits.
- Warranty: 2 years parts and labor.
- 30 day money back guarantee.
Operation – How do Column Packers Work?
Operationally, the pressure injection cell is analogous to a straw in a juice box. As you apply pressure in the box, the juice will be forced out through the straw. Likewise, the pressure injection cell is connected to pressurized gas, typically in a tank. A 0.5 to 2 mL tube containing the sample liquid is placed in the base of the pressure injection cell. A capillary is then placed through the ferrule in the cap and down into the sample tube. When packing a capillary, a frit assembly is placed at the distal end of the capillary to prevent particles from exiting and the entire pressure injection cell is typically placed on a stir plate. By regulating the gas pressure, you can adjust the flow rate of the sample into the capillary.
Products
Next Advance has been building column packers for many years and build a nice set of products around the packer. Below you find an overview of the different products and below that you find ancillary products like their Frit-Kit, allowing you to frit fused silica tubing to create columns.
| PC77 | PC8500 | PC1000 | |
| Pressure Rating | 2500 psi 170 Bar | 8500 psi 580 Bar | 1000 psi 69 Bar |
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| PC77 | PC77-MAG | PC8500 |
| Pressure Injection Cell is rated for 2500 psi (170 Bar). Includes a three-way valve for convenience. Includes a hex wrench, a frit kit, 10 ferrules for standard 360 µm OD capillaries and operator manual. Request your quote. | Pressure Injection Cell with integrated magnetic stir plate for keeping particles in suspension when packing capillaries. Also includes a 5x2mm stir bar, a hex wrench, frit kit, 10 ferrules for standard 360 µm OD capillaries and operator manual. Request your quote. | Pressure Injection Cell. High pressure version of PC77, rated for 8500 psi (580 Bar). Also includes a hex wrench, frit kit, 10 ferrules for standard 360 µm OD capillaries and operator manual. Request your quote. |
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| PC8500-MAG | PC1000 | PC1000-MAG |
| PC8500-MAG similar to PC77-MAG, except with a 8500 psi rating. This model has an integrated magnetic stir plate for keeping particles in suspension when packing capillaries. Also includes stir bar, a hex wrench, a frit kit, 10 ferrules for standard 360 µm OD capillaries and operator manual. Request your quote. | The PC1000 has a transparent body, so you can see the level of packing suspension in the vial. Rated for 1000 psi. Includes an integrated magnetic stir plate, a frit kit, a hex wrench, 10 ferrules for standard 360 µm OD capillaries and operator manual. Request your quote. | The PC1000-MAG has a transparent body, so you can see the level of packing suspension in the vial. Rated for 1000 psi. Includes an integrated magnetic stir plate, a frit kit, a hex wrench, 10 ferrules for standard 360 µm OD capillaries and operator manual. Request your quote. |
Click here to see all Column Packers in our online catalog, and to request your quote.
Ancillary products
Item
FRIT-KIT
Description
Frit Kit: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.
Request your quote.
CAP-75
Fused Silica Capillary Tubing: Capillary for LC/MS columns, 75 um ID, 363 um OD, 10 meter length (1 roll).
Request your quote.
CTOOL1
Capillary Cleaving Tool: Ceramic tool for cleaving fused silica capillary.
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PACK-KIT
The Column Packing Kit for use with the pressure injection cells includes vital accessories customers routinely purchase for setting up and using the pressure injection cell. Each kit includes HPREG high pressure regulator, TBNG10 stainless steel 1/8 inch diameter tubing, CAP-75 fused silica 75 µm inside diameter capillary and cleaving tool, and STRBR5X2 magnetic stir bar.
Request your quote.
PACK-KIT-3000
The Column Packing Kit for use with the pressure injection cells includes vital accessories customers routinely purchase for setting up and using the pressure injection cell. Each kit includes HP300 3000 psi high pressure regulator, TBNG10 stainless steel 1/8 inch diameter tubing, CAP-75 fused silica 75 µm inside diameter capillary and cleaving tool, and STRBR5X2 magnetic stir bar.
Request your quote.
For more information on Next Advance, click here.