Looking for a way to increase your LC-MS method accuracy for targeted protein quantification? Promise Proteomics has developed a solution to quality control your tryptic digestion, called DIGESTIF. Digestif is a new generic standard designed to release indicators peptides which reflect the efficency of the digestion step on any bottom-up experiment.
Promise Proteomics Digestif is an easy solution to Quality Control your Tryptic Digestion, Retention time reproducibility and Fragmentation efficiency by following these four steps:
|Digestif – Labelled 13C15N||Quality control of tryptic digestion||10 – 100 – 500 µg/tube||Request quote|
|Digestif – Unlabelled||Quality control of tryptic digestion||10 – 100 – 500 µg/tube||Request quote|
Promise’s Digestif can be used to standardize a broad spectrum of applications, ranging from simple replicate measurements up to large-scale biomarker screenings in biomedical applications.
- Digestif comprises a soluble recombinant protein scaffold + 11 artificial peptides (iRT) with good ionization.
- Amino acids flanking iRT cleavage site either favour or hinder protease cleavage.
- Retention time & relative intensity pattern of released iRT can assess the quality of sample workup, the extent of digestion, LC-MS performance & inter/intra-lab variability
- Reference: DIGESTIF: A Universal Quality Standard for the Control of Bottom-Up Proteomics Experiments. J Proteome Res. 2015 Feb 6;14(2):787-803
For more information on Promise Proteomics, click here.