Promise Proteomics Digestif is an easy solution to Quality Control your Tryptic Digestion, Retention time reproducibility and Fragmentation efficiency by following these four steps:
|Digestif – Labelled 13C15N||Quality control of tryptic digestion||10 – 100 – 500 µg/tube|
|Digestif – Unlabelled||Quality control of tryptic digestion||10 – 100 – 500 µg/tube|
- Digestif comprises a soluble recombinant protein scaffold + 11 artificial peptides (iRT) with good ionization.
- Amino acids flanking iRT cleavage site either favour or hinder protease cleavage.
- Retention time & relative intensity pattern of released iRT can assess the quality of sample workup, the extent of digestion, LC-MS performance & inter/intra-lab variability
- Reference: DIGESTIF: A Universal Quality Standard for the Control of Bottom-Up Proteomics Experiments. J Proteome Res. 2015 Feb 6;14(2):787-803
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