S-Trap Line

Setting the Industry Benchmark

S-Trap^™ sample processing begins with sample lysis and solubilization in 5% SDS. Proteins are further denatured by acidification to pH < 1 and subsequent exposure to a high concentration of methanol. This three-stage denaturation ensures complete destruction of undesired enzymatic activity such as proteases and phosphatases, and maximizes efficiency of downstream digestions. Reduction and alkylation can be performed in 5% SDS, precluding precipitation, or alternatively on-column after the denatured proteins are trapped.

Denatured, non-digested proteins are bound to the S-Trap via centrifugation, positive pressure or vacuum. Multiple weak-affinity interactions hold the undigested protein within the pores of the derivatized silica S-Trap. Captured proteins are presented with maximal surface area allowing them to be washed fully free of all contaminants in only minutes: detergents, PEG, glycerol, detergents, salts, Laemmli loading buffer, etc.

Upon addition of your choice of protease, physical confinement within the submicron pores of the trap forces substrate and protease interaction to yield rapid digestion. The trap does not have affinity for peptides, which elute after digestion.

S-Traps are available as spin columns, 96-well plates or full kits with premade buffers for convenience, minimal prep time and maximum reproducibility. 96-well plates are compatible with multiple automated platforms including the Tecan A200 positive pressure workstation and Agilent Bravo.

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S-Trap Columns

ProtiFi’s™ S-Trap™ columns are engineered for rapid, efficient, and reliable protein sample preparation across a wide variety of scales. Available in micro, mini, midi, & kit formats, these columns are designed to maximize recovery rates, ensure reproducibility, & consistently deliver high-quality results from small-scale experiments to comprehensive studies.

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S-Trap 96-Well Mini Plate

S-Trap™ 96-Well Mini Plates are designed for high-throughput applications and deliver consistent and reproducible results for all your studies. Engineered for compatibility with a wide range of fluid handlers, S-Trap™ 96-Well Mini Plates ensure reliable performance and exceptional sample integrity.

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S-Trap Kits

ProtiFi’s™ Universal Proteomics Sample Prep Kits streamline workflows, offering a complete suite of solutions for efficient, reproducible, and contaminant-free sample preparation. These kits are ideal for a diverse range of research scales and applications from small-scale exploratoration through complicated high-throughput, high-N studies.

Each kit includes all essential solutions and columns/plates to ensure you have everything required for efficient, reliable, and reproducible sample preparation. From initial protein extraction to final peptide cleanup, these components integrate seamlessly, streamlining your proteomics workflow and enhancing precision and consistency.

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